Training courses 2017: Training Course on RNA-Seq data analysis

IMPORTANT DATES for this Training course:

Deadline for applications: 30 June 2017
Course date: 18-20 July 2017

VENUE: Computer Room (aula Calcolo) Department of Biosciences, University of Milan (Via Celoria 26, Milan, Italy)

FEE: The course does not include a fee but the participants are expected to pay their own travel, meals and hotel costs (if any).

A maximum of 30 candidates will be accepted in the course. Selection will start on July 1st based on the candidates’ profile. Priority will be given to PhD students of the University of Milan and to candidates coming from abroad.

Instructors

Giulio Pavesi - Dept. of Biosciences, University of Milano
Fabrizio Ferré - Dept. of Pharmacy and Biotechnology, University of Bologna
Federico Zambelli - Dept. of Biosciences, University of Milano
Matteo Chiara - Dept. of Biosciences, University of Milano

Organisers

Giulio Pavesi (Dept. of Biosciences, University of Milano)
Federico Zambelli (Dept. of Biosciences, University of Milano and ELIXIR-IIB Technical Coordinator)
Matteo Chiara (Dept. of Biosciences, University of Milano)
Loredana Le Pera (ELIXIR-IIB Training Team)
Allegra Via (ELIXIR-IIB Training Coordinator, CNR, Italy)

Course Description

The course will provide students with theoretical and practical knowledge on how to perform bioinformatic analyses of RNA-Seq data, mainly aimed at the identification of genes differentially expressed across different conditions.

Target audience

PhD Students or young researchers in molecular biology and/or genetics with little or no background in bioinformatics.

Learning outcomes

After this course participants should be able to:

understand the theoretical principles of the analysis of RNA-Seq data
plan and execute the main steps of a bioinformatic RNA-Seq data analysis
use statistical approaches for the identification of differentially expressed genes
understand the results obtained

Course prerequisites

Basic background knowledge on genetics, molecular biology and transcriptomics.

Application Form

Programme

Tuesday - 18 July 2017
14:00 - 14:30	Opening and Welcome. Participants’ self-presentation
14:30 - 15:00	Lecture	Giulio Pavesi	Introduction to NGS and RNA-Seq
15:00 - 16:00	Lecture	Matteo Chiara	Theoretical principles in the analysis of RNA-Seq data.
16:00 - 16:30	Coffee break
16:30 - 17:30	Lecture	Federico Zambelli	Introduction to the Galaxy Platform
Wednesday - 19 July 2017
09:30 - 11:00	Practical	Fabrizio Ferré	Quality control, read mapping
11:00 - 11:30	Coffee break
11:30 - 13:00	Practical	Fabrizio Ferré	Read mapping and estimation of transcript levels
13:00 - 14:00	Lunch break
14:00 - 16:00	Practical	Fabrizio Ferré	Identification of differentially expressed genes
16:00 - 16:30	Coffe break
16:30 - 18:00	Practical	Fabrizio Ferré	Functional characterization of differentially expressed genes
Thursday - 20 July 2017
09:30 - 11:00	Lecture	Giulio Pavesi	Guidelines and strategies for post-processing of the results
11:00 - 12:00	Wrap-up and feedback