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Training courses: Biological networks: topological analysis and visualization using Cytoscape software

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Course Description

The study of the networks of protein-protein interactions within a cell, called “interactome”, provides information about the organization of the cell and allows us to identify proteins and signaling pathways that are disrupted or deregulated during a disease condition.
In this course, through a practical example, we will use the open-source software Cytoscape and some of its apps to extract protein-protein interaction data, build the network, visualize and explore it in order to detect topological properties, find potential topological clusters and perform functional network enrichment analysis.



Important Dates

  • Deadline for applications: 31st March 2026
  • Chosen participants will be notified by: 15th April 2026
  • Course date: 20th May 2026



Venue

University of Salerno Fisciano campus, Via Giovanni Paolo II, 132, 84084 Fisciano SA google maps link.



Fee

The course does not include a fee



Selection

A maximum of 30 candidates will be selected on a first-come- first-served basis. Selected participants will be notified by 15th April 2026.



Instructor

  • Luana Licata, University of Rome “Tor Vergata”, ELIXIR-IT, Italy



Helpers

  • Alessia Pinto, PhD student, University of Rome “Tor Vergata”, ELIXIR-IT, Italy

Organisers

  • Prof. Anna Marabotti, University of Salerno, ELIXIR-IT, Italy
  • Prof. Luana Licata - University of Rome “Tor Vergata”, ELIXIR-IT, Italy



Contact

For all kinds of queries, please contact the organizer Anna Marabotti or please write to Elixir_training_email.



Target audience

The course is for people participating to the meeting “Proteine 2026” Structural and functional characterization of proteins and their modulation.



Aims of the course

  • To provide practical demonstrations of several freely available protein-protein interactions resources.
  • To improve competences in protein-protein network analysis.
  • To improve the knowledge of Cytoscape and its network analysis and gene enrichment analysis tools.



Resources and tools covered

  • IMEx resources
  • STRING
  • Cytoscape
  • IntactApp
  • MCODE
  • ClueGo



Learning Outcomes

By the end of this course, the participants will be able to:

  • Identify and access public data resources and tools to study protein-protein interactions
  • Interpret the information given in different molecular interaction databases
  • Represent networks using Cytoscape
  • Perform network and functional analysis



Course prerequisites

Participants don’t need to have bioinformatics skills and previous knowledge of the tools that will be presented at the course. Attendees may bring in their own datasets for analysis, although example datasets will be available.



Registration

Please refer directly to the Web site https://www.mediacongress.it/congressi-in-corso/fisciano-proteins.html



Programme

Tuesday - 20 May

Time Learning Experience Topic
09:00-10:00 Lecture Introduction to Protein-Protein Interactions (PPIs) and Molecular Interaction Resources
10:00-10:30 Lecture Brief introduction to network biology and Cytoscape
10:30-10:45 Break
10:45-13:00 Exercise Building a molecular interaction network and analysing its topological features via Cytoscape.